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Plasmid Library

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Name Description Actions
pET-Cas9-NLS-6xHis Bacterial vector for regulated expression of S. pyogenes Cas9 with a C-terminal nuclear localization signal and 6xHis tag. View
pET-NLS-Cas9-6xHis Bacterial vector for regulated expression of S. pyogenes Cas9 with an N-terminal nuclear localization signal and C-terminal 6xHis tag. View
pFGC-pcoCas9 Binary plant vector for expressing plant codon-optimized Case9 from the 35SPPDK promoter. View
pGL3-U6-sgRNA-PGK-puromycin - View
pgRNA-bacteria Plasmid for expressing a single guide RNA (sgRNA) in bacteria. View
pgRNA-humanized Lentiviral vector for expressing a guide RNA (gRNA) together with a puromycin resistance marker and mCherry. View
pGuide - View
pGuide-it-sgRNA1 Vector (Linear) Linearized CRISPR/Cas9 vector with an improved scaffold, for cloning and expression of a single guide RNA (sgRNA). View
pGuide-it-tdTomato Vector (Linear) Linearized CRISPR/Cas9 vector with a tdTomato marker, for cloning and expression of a single guide RNA (sgRNA). View
pGuide-it-ZsGreen1 Vector (Linear) Linearized CRISPR/Cas9 vector with a ZsGreen1 marker, for cloning and expression of a single guide RNA (sgRNA). View
pHAGE EF1-alpha dCas9-KRAB Lentiviral CRISPR interference vector for constitutive expression of catalytically inactive dCas9 fused to a KRAB transcriptional repressor domain. View
pHAGE EF1-alpha dCas9-VP64 Lentiviral CRISPR activation vector for constitutive expression of catalytically inactive dCas9 fused to the VP64 transcriptional activation domain. View
pHAGE-TO-dCas9-3XGFP Lentiviral vector for labeling chromosomal loci by tetracycline-regulatable expression of catalytically inactive dCas9 fused to three copies of GFP. View
pHAGE-TO-dCas9-3XmCherry Lentiviral vector for labeling chromosomal loci by tetracycline-regulatable expression of catalytically inactive dCas9 fused to three copies of mCherry. View
pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2 Lentiviral vector for labeling chromosomal loci by tetracycline-regulatable expression of catalytically dead Streptococcus thermophilus dCas9 fused to three copies of mTagBFP2. View
pHAGE-TO-nmdCas9-3XGFP Lentiviral vector for labeling chromosomal loci by tetracycline-regulatable expression of catalytically dead Neisseria meningitidis dCas9 fused to three copies of GFP. View
pHAGE-TO-nmdCas9-3XmCherry Lentiviral vector for labeling chromosomal loci by tetracycline-regulatable expression of catalytically dead Neisseria meningitidis dCas9 fused to three copies of mCherry. View
pHAGE TRE dCas9 Lentiviral CRISPR activation vector for doxycycline-inducible expression of catalytically inactive dCas9. View
pHAGE TRE dCas9-KRAB Lentiviral CRISPR interference vector for doxycycline-inducible expression of catalytically inactive dCas9 fused to a KRAB transcriptional repressor domain. View
pHAGE TRE dCas9-VP64 Lentiviral CRISPR activation vector for doxycycline-inducible expression of catalytically inactive dCas9 fused to the VP64 transcriptional activation domain. View