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Plasmid Library

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Name Description Actions
pLenti-Cas9-P2A-tGFP 3rd-generation lentiviral vector for expressing Cas9 and TurboGFP under control of the CMV promoter. View
pLenti-Cas-Guide - View
pLenti-EF1a-Cas9-IRES-Puro 3rd-generation lentiviral vector for expressing Cas9 and a puromycin resistance marker under control of the EF-1α promoter. View
pLenti-Guide-Puro - View
pLKO.1-puro U6 sgRNA BfuAI stuffer - View
pLSC-5 - View
pLV hUbC-Cas9-T2A-GFP Lentiviral vector for co-expression of human codon-optimized Cas9 and enhanced GFP (EGFP). View
pLV hUbC-dCas9-T2A-GFP Lentiviral vector for expressing enhanced GFP (EGFP) together with catalytically inactive dCas9. View
pLV hUbC-dCas9 VP64-T2A-GFP Lentiviral vector for expressing enhanced GFP (EGFP) together with catalytically inactive dCas9 fused to the VP64 transcriptional activation domain. View
pLVX-hyg-sgRNA1 Vector (Linear) Linearized lentiviral vector with a hygromycin resistance marker, for cloning and expression of a single guide RNA (sgRNA). View
pLVX-puro-Cas9 Lentiviral Cas9 expression vector with the constitutive CMV promoter and a puromycin resistance marker. View
pLX-sgRNA - View
pMB66 C. elegans plasmid for expression of nematode codon-optimized Cas9 fused to monomeric EGFP and nuclear localization signals. View
pMB70 C. elegans plasmid for expressing a single guide RNA (sgRNA). View
pME-Cas9 Middle entry Gateway® vector encoding zebrafish codon-optimized S. pyogenes Cas9 with nuclear localization signals. View
pME-Cas9-T2A-GFP Middle entry Gateway® vector encoding zebrafish codon-optimized S. pyogenes Cas9 with nuclear localization signals and with a C-terminal GFP. View
pMJ806 pET-derived bacterial vector for expressing 6xHis- and MBP-tagged S. pyogenes Cas9. View
pMJ841 pET-derived bacterial vector for expressing 6xHis- and MBP-tagged catalytically inactive S. pyogenes Cas9. View
pMJ920 Doudna lab plasmid for mammalian cell expression of human codon-optimized Cas9 together with GFP. View
pMLM3705 CRISPR activation plasmid for mammalian cell expression of catalytically inactive dCas9 fused to the VP64 transcriptional activation domain. View