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Plasmid Library

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Name Description Actions
pMSCV-LTR-dCas9-p65AD-BFP Retroviral CRISPR activation vector for expressing TagBFP plus catalytically inactive dCas9 fused to the RelA (p65) transcriptional activation domain. View
pMSCV-LTR-dCas9-VP64-BFP Retroviral CRISPR activation vector for expressing TagBFP plus catalytically inactive dCas9 fused to the VP64 transcriptional activation domain. View
pMZ374 Fission yeast plasmid for expressing S. pyogenes Cas9 from the adh1 promoter plus a single guide RNA (sgRNA) from the rrk1 promoter/leader. View
pNW3 Plasmid for mammalian cell expression of the Csy4 CRISPR-associated endoribonuclease and a D10A nickase mutant of Cas9. View
pRGE31 Plant binary vector for transiently expressing a single guide RNA (sgRNA) together with Cas9. View
pRGEB31 Plant binary vector for stably expressing a single guide RNA (sgRNA) together with Cas9. View
pRGEB32 Plant vector for expressing a single guide RNA (sgRNA) with the rice U3 promoter and Cas9 with the rice ubiquitin promoter. View
pROSA26-Puro-DNR Mammalian donor vector with a puromycin resistance marker for transgene integration at the ROSA26 locus in the mouse genome. Use together with pCas‑Guide-ROSA26. View
pRPR1_gRNA_handle_RPR1t Yeast plasmid for expression of a guide RNA under control of the RNase P RNA (RPR1) promoter and terminator. View
pSECC Vector for expressing a single guide RNA (sgRNA) of interest together with Cas9 and Cre recombinase. View
pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) - View
pSLQ1658-dCas9-EGFP Retroviral vector encoding catalytically inactive dCas9 fused to enhanced GFP (EGFP), for visualizing genomic loci using a small guide RNA (sgRNA). View
pSpCas9(BB)-2A-GFP (PX458) - View
pSpCas9(BB)-2A-Puro (PX459) V2.0 - View
pSpCas9n(BB)-2A-GFP (PX461) - View
pSpCas9n(BB)-2A-Puro (PX462) V2.0 - View
pSpCas9n(BB) (PX460) - View
pSpCas9 (PX165) Zhang lab vector for expressing human codon-optimized Cas9. sgRNA sequences can be co-transfected as PCR products. View
pSPgRNA - View
pSQT1313 - View