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Plasmid Library

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Name Description Actions
pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP CRISPR activation vector encoding TagBFP plus catalytically inactive dCas9 fused to 10 copies of an optimized GCN4_v4 peptide. View
pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE CRISPR activation vector encoding TagBFP plus catalytically inactive dCas9 fused to 24 copies of an optimized GCN4_v4 peptide. View
pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS Vector encoding a single-chain anti-GCN4 peptide antibody fused to superfolder GFP, the VP64 activation domain, and the GB1 solubilization domain. View
pHR-SFFV-dCas9-BFP Lentiviral CRISPR interference vector for expressing catalytically inactive dCas9 fused to TagBFP. View
pHR-SFFV-dCas9-BFP-KRAB Lentiviral CRISPR interference vector for expressing catalytically inactive dCas9 fused to TagBFP and a KRAB domain. View
pHR-SFFV-KRAB-dCas9-P2A-mCherry Lentiviral CRISPR interference vector for expressing mCherry together with a KRAB domain fused to catalytically inactive dCas9. View
pHSN401 Plant vector for expressing a single guide RNA (sgRNA) together with maize codon-optimized Cas9. View
pHsp70-Cas9 Plasmid for expressing S. pyogenes Cas9 in Drosophila cells from the hsp70 promoter. Use together with pU6-BbsI-chiRNA. View
pICH41308_hCas9 Level 0 hCas9 module plasmid encoding human codon-optimized Cas9 with a nuclear localization signal. View
pICH47742_2x35S-5'UTR-hCas9(STOP)-NOST Level 1 hCas9 module plasmid for expressing human codon-optimized Cas9 from the CaMV 35S promoter. View
piCRg Entry - View
pICSL01009_AtU6p Level 0 vector encoding a consensus Arabidopsis U6 promoter for expressing a single guide RNA (sgRNA). View
pK7WGF2_hCas9 Plant vector for expressing human codon-optimized Cas9 with an N-terminal enhanced GFP (EGFP) tag. View
pKLV-U6gRNA(BbsI)-PGKpuro2ABFP - View
pL-CRISPR.EFS.GFP - View
pL-CRISPR.EFS.tRFP - View
pL-CRISPR.SFFV.GFP - View
pL-CRISPR.SFFV.tRFP - View
pLenti-Cas9 3rd-generation lentiviral vector for expressing Cas9 under control of the CMV promoter. View
pLenti-Cas9-IRES-Puro 3rd-generation lentiviral vector for expressing Cas9 and a puromycin resistance marker under control of the CMV promoter. View