Zettalab

Plasmid Library

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Name Description Actions
pCRCT High-copy yeast plasmid for disrupting genes by expressing S. pyogenes Cas9 together with a tracrRNA and one or more CRISPR RNAs (crRNAs). View
pCRISPomyces-1 Streptomyces plasmid for expression of codon-optimized Cas9, tracrRNA, and custom crRNA. View
pCRISPomyces-2 Streptomyces plasmid for expression of codon-optimized Cas9 and a single guide RNA (sgRNA). View
pCRISPR Plasmid for reconstituting the S. pyogenes CRISPR/Cas system in E. coli. Use together with pCas9. View
pCRISPRa-Enhancer Mammalian gene activation vector for expressing an MS2-p65-HSF1 fusion. Use together with pCas-Guide-CRISPRa. View
pCS2-Cas9 S. pyogenes Cas9 plasmid for in vivo expression or in vitro mRNA production. View
pCS2+hSpCas9 Plasmid for in vitro transcription of human codon-optimized S. pyogenes Cas9 using the SP6 promoter. View
pCS2-nCas9n Plasmid for expressing zebrafish codon-optimized S. pyogenes Cas9 with N- and C-terminal nuclear localization signals. View
pCW-Cas9 Lentiviral vector for doxycycline-inducible expression of FLAG®-tagged Cas9. View
pdCas9 Bacterial plasmid for expressing catalytically inactive dCas9 together with a crRNA precursor, for transcriptional repression. View
pdCas9-bacteria Bacterial plasmid for tetracycline-inducible expression of catalytically dead S. pyogenes Cas9. View
pdCas9 BFP-humanized Retroviral vector encoding human codon-optimized catalytically dead dCas9 fused to TagBFP, for visualizing genomic loci using a small guide RNA (sgRNA). View
pdCas9-humanized Retroviral vector encoding human codon-optimized catalytically dead dCas9 for CRISPR interference. View
pDCC6 Drosophila CRISPR/Cas9 genome editing vector for expressing a single guide RNA (sgRNA) together with Cas9. View
pDD162 (Peft-3_Cas9 + Empty sgRNA) C. elegans plasmid for expressing nematode codon-optimized Cas9 together with a single guide RNA (sgRNA). View
pDR274 Plasmid for in vitro transcription of a single guide RNA (sgRNA) using the T7 promoter. Archived by Addgene as DR274. View
Peft-3_cas9-SV40_NLS_tbb-2 3'UTR C. elegans plasmid for expressing nematode codon-optimized Cas9 with a synthetic intron. Use together with PU6::klp-12_sgRNA. View
pET28a Cas9-Cys Bacterial plasmid for regulated expression of S. pyogenes Cas9 with a C-terminal cysteine. View
pET-28b-Cas9-His Bacterial plasmid for expressing a 6xHis-tagged Cas9 protein, which can be purified and microinjected for gene editing. View
pET-Cas9-6xHis Bacterial vector for regulated expression of S. pyogenes Cas9 with a 6xHis tag. View