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Plasmid Library

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Name Description Actions
p415-GalL-Cas9-CYC1t Yeast centromeric plasmid for expressing human codon-optimized Cas9 with the inducible GAL1 promoter. View
p426-SNR52p-gRNA.CAN1.Y-SUP4t Yeast high-copy plasmid for gRNA targeting of the negatively selectable CAN1 gene. View
pAAV-Guide-it-1 Vector (Linear) Linearized CRISPR/Cas9 adeno-associated virus (AAV) vector with S. aureus Cas9, for cloning and expression of a single guide RNA (sgRNA). View
pAAV-Guide-it-Down Vector (Linear) CRISPR/Cas9 adeno-associated virus (AAV) vector for cloning and expression of a single guide RNA (sgRNA). Use together with pAAV-Guide-it-Up Vector. View
pAAV-Guide-it-Up Vector CRISPR/Cas9 adeno-associated virus (AAV) vector for cloning and expression of a single guide RNA (sgRNA). Use together with pAAV-Guide-it-Down Vector. View
pAAVS1-BSD-DNR Mammalian donor vector with a blasticidin resistance marker for transgene integration at the AAVS1 locus in the human genome. Use together with pCas-Guide-AAVS1. View
pAAVS1-Puro-DNR Mammalian donor vector with a puromycin resistance marker for transgene integration at the AAVS1 locus in the human genome. Use together with pCas-Guide-AAVS1. View
pAC149-pCR8-dCas9VP160 Gateway® entry vector with a cassette encoding catalytically inactive dCas9 fused to the VP160 transcriptional activation domain. View
pAC152-dual-dCas9VP64-sgExpression - View
pAC153-dual-dCas9VP96-sgExpression - View
pAC154-dual-dCas9VP160-sgExpression - View
pAC155-pCR8-sgExpression - View
pAC2-dual-dCas9VP48-sgExpression - View
pAC84-pCR8-dCas9 Gateway® entry vector with a cassette encoding HA epitope-tagged catalytically inactive S. pyogenes dCas9. View
pAC91-pmax-dCas9VP64 CRISPR activation vector for expressing catalytically inactive dCas9 fused to the VP64 transcriptional activation domain. View
pAC93-pmax-dCas9VP160 CRISPR activation vector for expressing catalytically inactive dCas9 fused to the VP160 transcriptional activation domain. View
pAC94-pmax-dCas9VP160-2A-puro CRISPR activation vector for expressing a puromycin resistance marker plus catalytically inactive dCas9 fused to the VP160 transcriptional activation domain. View
pAC95-pmax-dCas9VP160-2A-neo CRISPR activation vector for expressing a G418 resistance marker plus catalytically inactive dCas9 fused to the VP160 transcriptional activation domain. View
pAc-sgRNA-Cas9 Plasmid for expressing a single guide RNA (sgRNA) together with Cas9 in Drosophila cells. View
pACT2-CAS9c High-copy yeast plasmid for expressing S. pyogenes Cas9 under control of the ADH1 promoter and terminator. View