Zettalab

Plasmid Library

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Name Description Actions
pBS-Hsp70-Cas9 Bacterial plasmid encoding S. pyogenes Cas9 under control of the Drosophila hsp70 promoter and poly(A) signal. View
pCAG-T3-hCasD10A-pA Plasmid for mammalian cell expression or in vitro transcription of human codon-optimized Cas9 with the D10A nickase mutation. View
pCAG-T3-hCAS-pA Plasmid for mammalian cell expression or in vitro transcription of human codon-optimized Cas9. View
pCas Bacterial plasmid with a temperature-sensitive replicon encoding Cas9, an inducible single guide RNA (sgRNA), and the λ Red recombination system. View
pCas9 Plasmid for reconstituting the S. pyogenes CRISPR/Cas system in E. coli. Use together with pCRISPR. View
pCas9D10A_GFP Plasmid for co-expression in mammalian cells of enhanced GFP (EGFP) and human codon-optimized Cas9 with the D10A nickase mutation. View
pCas9_GFP Plasmid for co-expression in mammalian cells of enhanced GFP (EGFP) and human codon-optimized Cas9. View
pCas-Guide - View
pCas-Guide-AAVS1 Mammalian guide RNA (gRNA) vector for transgene integration at the AAVS1 locus in the human genome. Use together with pAAVS1-Puro-DNR. View
pCas-Guide-CRISPRa - View
pCas-Guide-CRISPRi - View
pCas-Guide-EF1a-CD4 - View
pCas-Guide-EF1a-GFP - View
pCas-Guide-Nickase - View
pCas-Guide-ROSA26 Mammalian guide RNA (gRNA) vector for transgene integration at the ROSA26 locus in the mouse genome. Use together with pROSA26-Puro-DNR. View
pcDNA3.1-CibN-dCas9-CibN Plasmid encoding two copies of CIBN fused to catalytically inactive dCas9, for use in the light-activated CRISPR-Cas9 effector (LACE) system. View
pcDNA-dCas9 Plasmid for expressing catalytically inactive dCas9 in mammalian cells using the CMV promoter. View
pcDNA-dCas9-p300 Core Mammalian cell plasmid for CRISPR activation by expressing catalytically inactive dCas9 fused to the p300 core histone acetyltransferase domain. View
pcDNA-dCas9-p300 Core (D1399Y) Control plasmid for CRISPR activation, encoding catalytically inactive dCas9 plus an inactivated form of the p300 core transcriptional activation domain. View
pcDNA-dCas9-VP64 Mammalian cell plasmid for CRISPR activation by expressing catalytically inactive dCas9 fused to the VP64 transcriptional activation domain. View