Zettalab

Plasmid Library

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Name Description Actions
lentiCas9-EGFP Zhang lab lentiviral vector for expressing human codon-optimized S. pyogenes Cas9 plus enhanced GFP (EGFP). View
lentiCas9n(D10A)-Blast Zhang lab lentiviral vector with a blasticidin resistance marker, for expressing Cas9 with the D10A nickase mutation. View
lentiCRISPR v2 Zhang lab lentiviral transfer plasmid, also known as pLentiCRISPR v2, for CRISPR/Cas9 genome editing of mammalian cells with a single guide RNA (sgRNA). View
lenti dCAS-VP64_Blast Zhang lab lentiviral vector encoding a blasticidin resistance marker plus catalytically inactive dCas9 fused to the VP64 transcriptional activation domain. View
lentiGuide-Puro Zhang lab lentiviral vector, also known as pLentiGuide-Puro, for expressing a single guide RNA (sgRNA) in Cas9-expressing cells. Use together with lentiCas9-Blast. View
lenti MS2-P65-HSF1_Hygro Zhang lab lentiviral vector for expressing a hygromycin resistance marker plus an MS2 RNA-binding domain fused to the p65 and HSF1 transcription factors. View
lenti sgRNA(MS2)_zeo backbone Zhang lab lentiviral vector for expressing a Zeocin™-resistance marker plus a single guide RNA (sgRNA) containing two MS2 stem loops. View
LSL-Cas9-Rosa26TV Zhang lab vector with positive and negative selection markers, for targeting Cre-dependent Cas9 and EGFP expression to the mouse Rosa26 locus. View
MLM3613 Plasmid for expressing S. pyogenes Cas9 from either the CMV promoter in vivo or the T7 promoter in vitro. View
MLM3636 - View
MLM3639 Plasmid for expressing 3xFLAG®-tagged S. pyogenes Cas9 from either the CMV promoter in vivo or the T7 promoter in vitro. View
MS2-P65-HSF1_GFP Zhang lab vector for expressing GFP together with an MS2 RNA-binding domain fused to the p65 and HSF1 transcription factors. View
MSP1101 Vector for expressing the S. pyogenes Cas9 VRER mutant, which has an altered PAM recognition specificity. View
MSP2133 Mammalian cell plasmid for expressing the high-fidelity S. pyogenes Cas9 variant SpCas9-HF4. View
MSP2135 Mammalian cell plasmid for expressing the high-fidelity S. pyogenes Cas9 variant SpCas9-HF2. View
MSP469 Vector for expressing the S. pyogenes Cas9 VQR mutant, which has an altered PAM recognition specificity. View
M-SPcas Mammalian cell vector for expressing human codon-optimized Streptococcus pyogenes Cas9 with a G418-resistance marker. View
M-ST1cas Mammalian cell vector for expressing human codon-optimized Streptococcus thermophilus Cas9 with a G418-resistance marker. View
p3s-Cas9HC Mammalian cell vector for expressing S. pyogenes Cas9 with a C-terminal HA epitope tag. View
p414-TEF1p-Cas9-CYC1t Yeast centromeric plasmid for expressing human codon-optimized Cas9 with the constitutive TEF1 promoter. View