Plasmid Library
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Name | Description | Actions |
---|---|---|
lac operator | DNA sequence element that binds the lac repressor. | View |
lac promoter | Promoter for the E. coli lac operon. | View |
lac UV5 promoter | E. coli lac promoter with an "up" mutation. | View |
lacZ | E. coli lacZ gene encoding β-galactosidase. | View |
Lafmid BA | Phagemid vector used for cDNA cloning. | View |
lambda | Complete annotated DNA sequence of bacteriophage lambda, including the cohesive ends. | View |
LanYFP | Tetrameric yellow fluorescent protein from Branchiostoma lanceolatum, also known as blFP-Y3. | View |
lentiCas9-Blast | Zhang lab lentiviral vector with a blasticidin resistance marker, also known as pLentiCas9-Blast, for expressing Cas9. Use together with lentiGuide-Puro. | View |
lentiCas9-EGFP | Zhang lab lentiviral vector for expressing human codon-optimized S. pyogenes Cas9 plus enhanced GFP (EGFP). | View |
lentiCas9n(D10A)-Blast | Zhang lab lentiviral vector with a blasticidin resistance marker, for expressing Cas9 with the D10A nickase mutation. | View |
lentiCRISPR v2 | Zhang lab lentiviral transfer plasmid, also known as pLentiCRISPR v2, for CRISPR/Cas9 genome editing of mammalian cells with a single guide RNA (sgRNA). | View |
lenti dCAS-VP64_Blast | Zhang lab lentiviral vector encoding a blasticidin resistance marker plus catalytically inactive dCas9 fused to the VP64 transcriptional activation domain. | View |
lentiGuide-Puro | Zhang lab lentiviral vector, also known as pLentiGuide-Puro, for expressing a single guide RNA (sgRNA) in Cas9-expressing cells. Use together with lentiCas9-Blast. | View |
lenti MS2-P65-HSF1_Hygro | Zhang lab lentiviral vector for expressing a hygromycin resistance marker plus an MS2 RNA-binding domain fused to the p65 and HSF1 transcription factors. | View |
lenti sgRNA(MS2)_zeo backbone | Zhang lab lentiviral vector for expressing a Zeocin™-resistance marker plus a single guide RNA (sgRNA) containing two MS2 stem loops. | View |
LITMUS28 | High copy-number E. coli vector with a multiple cloning site flanked by mutant T7 promoters. For wild-type T7 promoters, use LITMUS28i. | View |
LITMUS28i | High copy-number E. coli vector with a multiple cloning site flanked by wild-type T7 promoters. For an alternative MCS, use LITMUS38i. | View |
LITMUS29 | High copy-number E. coli vector with a multiple cloning site flanked by mutant T7 promoters. The MCS is reversed relative to LITMUS28. | View |
LITMUS38 | High copy-number E. coli vector with a multiple cloning site flanked by T7 promoters. For wild-type T7 promoters, use LITMUS38i. | View |
LITMUS38i | High copy-number E. coli vector with a multiple cloning site flanked by wild-type T7 promoters. For an alternative MCS, use LITMUS28i. | View |