Plasmid Library
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| Name | Description | Actions |
|---|---|---|
| pBluescript-FL | cDNA cloning vector derived from pBluescript II KS(-) by replacing the SmaI site with a fragment containing two PflMI sites. | View |
| pBluescript II KS(-) | Standard cloning vector (phagemid excised from lambda ZAPII). The f1 (–) orientation allows rescue of antisense strand ssDNA. pBluescript II SK(–) has a reversed MCS. | View |
| pBluescript II KS(+) | Standard cloning vector (phagemid excised from lambda ZAPII). The f1 (+) orientation allows rescue of sense strand ssDNA. pBluescript II SK(+) has a reversed MCS. | View |
| pBluescript II KS(+) | - | View |
| pBluescript II SK(-) | Standard cloning vector (phagemid excised from lambda ZAPII). The f1 (–) orientation allows rescue of antisense strand ssDNA. pBluescript II KS(–) has a reversed MCS. | View |
| pBluescript II SK(+) | Standard cloning vector (phagemid excised from lambda ZAPII). The f1 (+) orientation allows rescue of sense strand ssDNA. pBluescript II KS(+) has a reversed MCS. | View |
| pBluescript II SK(+) | Standard cloning vector (phagemid excised from lambda ZAPII). The f1 (+) orientation allows rescue of sense strand ssDNA. pBluescript II KS(+) has a reversed MCS. | View |
| pBluescript KS(-) | Standard cloning vector (phagemid excised from lambda ZAP). The f1 (–) orientation allows rescue of antisense strand ssDNA. pBluescript SK(–) has a reversed MCS. | View |
| pBluescript KS(+) | Standard cloning vector (phagemid excised from lambda ZAP). The f1 (+) orientation allows rescue of sense strand ssDNA. pBluescript SK(+) has a reversed MCS. | View |
| pBluescript KS(+) | Standard cloning vector (phagemid excised from lambda ZAP). The f1 (+) orientation allows rescue of sense strand ssDNA. pBluescript SK(+) has a reversed MCS. | View |
| pBluescriptR | pBluescript derivative modified at RIKEN for cDNA cloning. | View |
| pBluescript SK(-) | Standard cloning vector (phagemid excised from lambda ZAP). The f1 (–) orientation allows rescue of antisense strand ssDNA. pBluescript KS(–) has a reversed MCS. | View |
| pBluescript SK(-) | Standard cloning vector (phagemid excised from lambda ZAP). The f1 (-) orientation allows rescue of antisense strand ssDNA. | View |
| pBluescript SK(+) | Standard cloning vector (phagemid excised from lambda ZAP). The f1 (+) orientation allows rescue of sense strand ssDNA. pBluescript KS(+) has a reversed MCS. | View |
| pBluescript SK(+) | Standard cloning vector (phagemid excised from lambda ZAP). The f1 (+) orientation allows rescue of sense strand ssDNA. pBluescript KS(+) has a reversed MCS. | View |
| pBlue-TOPO (linearized) | Linearized vector with 3''-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products to measure promoter activity using β-galactosidase. | View |
| pBlue-TOPO (linearized) | Linearized vector with 3''-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products to measure promoter activity using β-galactosidase. | View |
| pBR322 | First-generation E. coli vector for DNA cloning. This plasmid has a low copy number (~20 copies per cell) due to the rop gene. | View |
| pBridge | Yeast three-hybrid vector for expressing a "bait" protein fused to the GAL4 DNA-binding domain, plus an additional bridge protein. | View |
| pB-RS | HuSH-29 retroviral vector with ampicillin and blasticidin resistance markers, for expressing an shRNA from the U6 promoter. | View |