Plasmid Library
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Name | Description | Actions |
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pCR4Blunt-TOPO (linearized) | Linearized vector with bound topoisomerase, for TOPO® cloning of blunt PCR products. Can be used to generate nested deletions for sequencing. | View |
pCR4-TOPO (linearized) | Linearized vector with 3''-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products. Can be used to generate nested deletions for sequencing. | View |
pCR8 GW TOPO (linearized) | Linearized vector with 3''-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products to create a Gateway® entry vector. | View |
pCR-Blunt II-TOPO (linearized) | - | View |
pCRII-TOPO (linearized) | Linearized vector with 3''-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products. Similar to pCR®2.1-TOPO® but with an SP6 promoter. | View |
pCR-XL-2-TOPO (linearized) | Linearized vector with bound topoisomerase, for TOPO® cloning of long blunt-ended PCR products. Positive clones disrupt a lethal lacZα-ccdB fusion gene. | View |
pCR-XL-TOPO (linearized) | Linearized vector with 3''-T overhangs and bound topoisomerase, for TOPO® TA cloning of long PCR products. Positive clones disrupt a lethal lacZα-ccdB fusion gene. | View |
pEF5 FRT V5-D-TOPO | Directional TOPO® cloning vector for constitutive high-level expression of proteins in mammalian cells using the Flp-In™ system. | View |
pEF6 V5-His-TOPO (linearized) | Linearized vector with 3''-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products and expression of tagged proteins in mammalian cells. | View |
pENTR5'-TOPO (linearized) | Linearized vector with 3''-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products to create a multisite Gateway® entry clone. | View |
pENTR D-TOPO | Directional TOPO® cloning vector, for creating a Gateway® entry vector that is suitable for recombination with a destination vector. | View |
pENTR SD D-TOPO | Directional TOPO® cloning vector, for creating a Gateway® entry vector that is optimal for recombination with a prokaryotic destination vector. | View |
pENTR TEV D-TOPO | Directional TOPO® cloning vector with a TEV protease site, for creating a Gateway® entry vector that is suitable for recombination with a destination vector. | View |
pET100 D-TOPO | Bacterial directional TOPO® cloning vector with an ampicillin resistance marker, for inducible expression of an N-terminally 6xHis-tagged protein. | View |
pET101 D-TOPO | Bacterial directional TOPO® cloning vector, for inducible expression of a C-terminally V5- and 6xHis-tagged protein. | View |
pET102 D-TOPO | Bacterial directional TOPO® cloning vector, for tagging a protein N-terminally with His-Patch thioredoxin and C-terminally with V5 and 6xHis. | View |
pET151 D-TOPO | Bacterial directional TOPO® cloning vector, for inducible expression of an N-terminally 6xHis- and V5-tagged protein. | View |
pET160 GW D-TOPO | Bacterial directional TOPO® cloning vector, for generating a Gateway® expression clone with N-terminal 6xHis and tetracysteine tags. | View |
pET161 GW D-TOPO | Bacterial directional TOPO® cloning vector, for generating a Gateway® expression clone with C-terminal tetracysteine and 6xHis tags. | View |
pET200 D-TOPO | Bacterial directional TOPO® cloning vector with a kanamycin resistance marker, for inducible expression of an N-terminally 6xHis-tagged protein. | View |